Shortscript.io. sgRNAs were generated by MEGA shortscript T7 in vitro transcription (Thermo Fisher; AM1354) according to standard methods (performed in-house or purchased directly from Thermo Fisher). Shortscript.io

 
sgRNAs were generated by MEGA shortscript T7 in vitro transcription (Thermo Fisher; AM1354) according to standard methods (performed in-house or purchased directly from Thermo Fisher)Shortscript.io  Step 2: Creating Creative Hook Angles

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